Open Positions: Research Scientist, Cellular and Molecular Biologist, Oncology
Arvinas is a pharmaceutical company focused on developing new small molecules ‒ known as PROTACs (PROteolysis TArgeting Chimeras) ‒ aimed at degrading disease-causing cellular proteins via proteolysis. Based on innovative research conducted at Yale University by Dr. Craig Crews, Founder and Chief Scientific Advisor, the company is translating natural protein degradation approaches into novel drugs for the treatment of cancer and other diseases. The proprietary PROTAC-based drug paradigm induces protein degradation, rather than protein inhibition, using the ubiquitin proteasome system and offers the advantage of potentially targeting “undruggable” as well as “druggable” elements of the proteome. This greatly expands the ability to create drugs for many new, previously unapproachable targets. For more information, visit www.arvinas.com.
This position is laboratory/wet bench based and requires strong experimental technique, the ability to deliver quality data in a timely manner from high throughput cell-based assays and work effectively with team members in support of small molecule drug discovery efforts. Data analysis and interpretation is required, as will be method development where appropriate.
This position reports to a Research Investigator within the Biology department and will be located at our headquarters in New Haven, CT.
Key responsibilities of this role include, but are not limited to:
- Design, execute, analyze and interpret cell-based experiments using various cell culture plate formats with suspension and adherent cell lines
- Maintainence of cell lines used in own experiments, ensuring QC of and LN stocks are maintained and catalogued appropriately
- Conduct monthly screening of small molecules by FRET based analyses and ensure database entry in a timely manner
- Leverage multiple assay platforms to measure, understand and interpret target engagement, pathway modulation and cell sensitivity in response to small molecule treatment/target modulation
- Perform molecular biology assays utilizing DNA/RNA isolation, PCR, RT-PCR, qRT-PCR and gene expression methodologies
- Identify and characterize appropriate cell models that are dependent on the target of interest
- Bacterial or mammalian cell line engineering such as cloning, shRNA or siRNA transfection, or lentiviral infection, as needed
- Utilize compounds and molecular target manipulation (e.g. RNAi) to dissect target function and dependency
- Interact and collaborate with biology and chemistry team members to contribute to drug discovery and development program progression
- Present data at regular team meetings
- Document, compile and manage laboratory notebook, databases, spreadsheets, or other data files to present and collect data in a meaningful manner
- Keep up-to-date on relevant scientific literature
- Conduct experiments and interact with team members in the fashion of a good lab colleague
- Proficiency in aseptic tissue culture techniques and mammalian cell tissue culture is essential
- Experience with protein lysate preparation from cells and tissues, semi-quantitative Western blotting
- Molecular techniques including DNA/RNA isolation, cloning, and PCR/RT-PCR/qRT-PCR is required
- Experience in cellular functional assays such as cytotoxicity, apoptosis, cell cycle analysis, growth inhibition, and siRNA/shRNA transfection is preferred
- Experience working with small molecules to generate full dose response curves in drug discovery using FRET or similar biochemical method is preferred
- Development, optimization of, or experience with high throughput (96/384 well) cell-based assays using a variety of readout technologies (e.g. IF, fluorescence, luminescence, ELISA, AlphaLISA, MSD) is preferred
- Excellent data handling and recording skills as well as oral and written communication skills is required
- Ability to work as a team player, able to multitask and change direction quickly is required
- General knowledge of cancer biology is preferred
- Proficiency in execution and data analysis in the following is a plus;
- IP, ChIP
- High content IF cell imaging
- Flow cytometry
- 2D/3D cellular proliferation assays
- CRISPR knockdown assays
- Organized, goal-oriented and timeline-conscious with the ability to plan, work and problem-solve independently while under general supervision by the project leader
- A strong work ethic and willingness to learn drug discovery approaches and apply them in cell biology and other techniques is required.
- PhD in Biology or relevant discipline with > 2 yrs post-doc or industry experience post-PhD
Arvinas is proud to offer a competitive package of base and incentive compensation as well as a comprehensive benefits program designed to support the health, wellness, and financial security of our employees and their families. Benefits include group medical, vision, and dental coverage, group and supplemental life insurance, and much more. To learn more about Arvinas, please visit www.arvinas.com.
Arvinas is an Equal Opportunity Employer.